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Promega
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FUJIFILM
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Merck KGaA
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Informa UK Limited
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Macklin Inc
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Shanghai Macklin Biochemical
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Manan Medical Products Inc
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Image Search Results
Journal: Archives of biochemistry and biophysics
Article Title: UGDH promotes 5-fluorouracil resistance in colorectal cancer via the ROS-activated PI3K/AKT-EEF1A2-PRDX1 pathway.
doi: 10.1016/j.abb.2025.110445
Figure Lengend Snippet: Fig. 7. ROS activate the PI3K/AKT pathway, regulating the UGDH-EEF1A2-PRDX1 axis to promote chemoresistance. (A) KEGG pathway enrichment of DEGs in UGDH-knockdown SW620 cells (n = 3 replicates). (B) Western blot of PI3K/AKT pathway proteins in HCT8 cells ± LY294002 (10 μM, 48 h, n = 3). (C) PI3K/AKT phosphorylation in HCT8 control and shUGDH cells ± 5-FU (n = 3). (D) UGDH and PI3K/AKT activation in HCT8 cells treated with H2O2 (0–200 μM, 24 h, n = 3). (E) Time-dependent UGDH activation in HCT8 cells treated with 15 μM 5-FU (0–96 h). (n = 3). (F) Western blot of PI3K/AKT pathway proteins in HCT8 cells ± LY294002 (10 μM, n = 3)/± NAC (750 μМ, n = 3). Data are mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Article Snippet: The primary antibodies used were against
Techniques: Knockdown, Western Blot, Phospho-proteomics, Control, Activation Assay